“According to a new report published by Introspective Market Research, Short-read Sequencing Market by Technology, Application, and End User, The Global Short-read Sequencing Market Size Was Valued at USD 7.50 Billion in 2024 and is Projected to Reach USD 48.98 Billion by 2035, Growing at a CAGR of 18.6% from 2025 to 2035.”

The short-read sequencing market plays a critical role in modern genomics by enabling high-throughput, accurate, and cost-effective DNA and RNA sequencing. This technology generates short DNA fragments that are sequenced rapidly and assembled using advanced bioinformatics tools. Compared to traditional sequencing methods, short-read sequencing offers superior scalability, reproducibility, and efficiency, making it the preferred choice for large-scale genomic studies.

Short-read sequencing is widely adopted across research institutes, clinical diagnostics, pharmaceutical companies, and biotechnology firms. Its applications span whole-genome sequencing, transcriptomics, epigenetics, oncology research, and infectious disease analysis. The technology’s ability to process millions of reads simultaneously supports population-scale studies and precision medicine initiatives.

Continuous advancements in sequencing platforms, automation, and data analysis software are further enhancing performance while reducing turnaround time and costs. As genomics becomes central to personalized healthcare and drug discovery, short-read sequencing remains a foundational technology driving innovation across life sciences.

Market Segmentation

The Short-read Sequencing Market is segmented into Technology, Application, and End User.
By Technology, the market is categorized into Sequencing by Synthesis, Sequencing by Ligation, and Ion Semiconductor Sequencing.
By Application, the market is categorized into Whole Genome Sequencing, Targeted Sequencing, RNA Sequencing, and Epigenetic Sequencing.
By End User, the market is categorized into Academic & Research Institutes, Hospitals & Diagnostic Centers, Pharmaceutical & Biotechnology Companies, and Contract Research Organizations.

Growth Driver

The primary growth driver of the short-read sequencing market is the rising adoption of genomics in clinical diagnostics and precision medicine. Increasing prevalence of cancer, genetic disorders, and infectious diseases has accelerated the demand for accurate and rapid sequencing solutions. Short-read sequencing enables large-scale genomic analysis with high accuracy, supporting early disease detection and personalized treatment strategies. Additionally, declining sequencing costs and growing government funding for genomic research initiatives are encouraging widespread adoption across healthcare and research settings, significantly boosting market growth.

Market Opportunity

A major opportunity in the short-read sequencing market lies in its expanding use in population genomics and large-scale screening programs. Governments and research organizations worldwide are launching national genomics initiatives to study genetic variations across populations. Short-read sequencing, due to its scalability and cost-effectiveness, is ideally suited for such programs. Furthermore, integration of artificial intelligence and advanced bioinformatics tools with sequencing platforms presents new opportunities for data interpretation, enabling faster insights and driving broader adoption across emerging markets.

Detailed Segmentation

Short-read Sequencing Market, Segmentation

The Short-read Sequencing Market is segmented on the basis of Technology, Application, and End User.

Technology

The Technology segment is further classified into Sequencing by Synthesis, Sequencing by Ligation, and Ion Semiconductor Sequencing. Among these, the Sequencing by Synthesis sub-segment accounted for the highest market share in 2024. This dominance is attributed to its high accuracy, scalability, and widespread adoption in both research and clinical applications. The technology supports high-throughput sequencing and is compatible with a wide range of genomic workflows, making it the preferred choice among end users.

Application

The Application segment is further classified into Whole Genome Sequencing, Targeted Sequencing, and RNA Sequencing. Among these, the Whole Genome Sequencing sub-segment held the largest market share in 2024. Its ability to provide comprehensive genomic information supports advanced research, disease diagnosis, and personalized medicine initiatives. Increasing demand for complete genomic insights in oncology and rare disease research continues to drive growth in this segment.

Some of The Leading/Active Market Players Are-

• Illumina, Inc. (USA)
• Thermo Fisher Scientific Inc. (USA)
• Agilent Technologies, Inc. (USA)
• BGI Genomics (China)
• Roche Diagnostics (Switzerland)
• QIAGEN N.V. (Netherlands)
• Pacific Biosciences (USA)
• Oxford Nanopore Technologies (UK)
• PerkinElmer, Inc. (USA)
• Eurofins Scientific (Luxembourg)
• Macrogen, Inc. (South Korea)
• GenScript Biotech Corporation (USA)
• Bio-Rad Laboratories, Inc. (USA)
• Takara Bio Inc. (Japan)
• LGC Limited (UK)
and other active players.

Key Industry Developments

In March 2024, a leading sequencing technology provider launched an advanced short-read sequencing platform with enhanced throughput and reduced turnaround time.
The new platform is designed to support large-scale clinical and research applications, offering improved accuracy, automation, and cost efficiency, strengthening the company’s competitive position.

In September 2024, a major genomics research initiative expanded its use of short-read sequencing for population-scale genetic studies.
This expansion aims to accelerate disease research and personalized medicine by generating high-quality genomic data across diverse population groups.

Key Findings of the Study

• Sequencing by synthesis remains the dominant technology segment
• North America leads the market due to strong research infrastructure
• Rising demand for precision medicine is a key growth driver
• Whole genome sequencing is the leading application segment
• Technological advancements continue to reduce sequencing costs

More Info: https://introspectivemarketresearch.com/reports/short-read-sequencing-market/

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